Custom primers can be used on the AVITI either through a spike-in or a replacement method. The spike-in method allows users to directly add custom primer(s) to the primer tubes in the sequencing cartridge. The replacement method allows users to add primers to custom buffer tubes and replace the sequencing primer vial tubes in the reagent cartridge with the tubes containing the custom primer(s).
Customers using CB Freestyle Sequencing Kits will use the CB Freestyle sequencing primer vials for the spike-in method or the Custom Primer Set Cloudbreak Freestyle for the replacement method.
Customers using Cloudbreak Sequencing Kits will spike-in primers to the Adept Custom Primer Set Cloudbreak or use the Adept Custom Primer Set Cloudbreak for the replacement method.
Full instructions for the use of custom primers can be found in the Element AVITI System User Guide.