Trinity

The traditional hybrid selection process for targeted panels, like exomes, involves a time-consuming process with multiple temperature-controlled washes, PCR-based amplification, and library quality control.

With the simplified Trinity workflow, you simply hybridize your DNA library to the target probes of interest, wait for the prescribed time, and load it directly onto an AVITI—drastically reducing manual steps and additional amplification resulting in higher library complexity and lower duplication rates.

Learn more aobut the Trinity Workflow

We have established supported workflows for exome sequencing applications in collaboration with Twist Bioscience and IDT. Trinity sequencing kits and binding reagents can be purchased directly from Element Biosciences while reagents for library preparation and hybridization can be purchased from either of our partners.

Exome sequencing is our first supported application, but with some optimization Trinity sequencing kits are compatible with panels ranging from hundreds to thousands of targets. Please contact us for additional guidance on running alternative panels.

Learn more about Trinity compatibility

Trinity not only improves the workflow of hybrid selection sequencing, it also improves performance with a dramatic reduction in duplication rate and higher library complexity when compared to libraries run in-solution. This allows for more efficient sequencing where we see higher coverage per sequencing read.

Download our infographic to see the data

Trinity sequencing kits are configured to sequence 24 exomes per flow cell with mean target coverage at ≥ 50x and the ability to scale the plexity depending on your coverage needs. For added TAT flexibility, Trinity is available in both 2 x 75 and 2 x 150 options.