Element Adept™ Library Compatibility Workflow

The Adept Workflow makes libraries prepared with third-party kits compatible with the Element AVITI™ System without changes to upstream library prep or analysis. Simply augment your existing sequencing workflow with AVITI to capitalize on cost, flexibility, and quality for any project.

Custom splint oligos kitted with circularization or amplification reagents unlock a diversity of applications, from exomes to whole-genome sequencing (WGS). Multiple kit and protocol options ensure library compatibility and choice of key experiment parameters.

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Adept Library Compatibility Workflow

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ProtocolElement KitCompatible InputFinal LibraryCircularization MethodQuantification Method

Adept Standard

Element Adept Library Compatibility Kit v1.1

Reach out to Element Tech Support for a full list

CircularOn-benchqPCR

Adept Rapid PCR-Free

Element Adept Library Compatibility Kit v1.1

Reach out to Element Tech Support for a full listCircularOn-benchqPCR

Adept Rapid PCR-Plus

Adept Rapid PCR-Plus Kit

Any libraryLinearOnboardQubit

The Element Adept Library Compatibility Kit v1.1 (Catalog #830-00007) offers benchtop circularization of libraries prior to sequencing. We have verified compatibility of this kit with many 3rd party sequencing assays. For complete circularization, all assays require full-length libraires without 5’ or 3’ additions, truncations, or modifications. Please reach out to your Field Applications Scientist or Element Tech Support for more information.

Kit Specifications

The Element Adept Library Compatibility Kit v1.1 and Adept Rapid PCR-Plus supply familiar reagents and procedures that are complete in as little as 40 minutes. Depending on protocol, you can manually circularize libraries on-bench or amplify the libraries for automatic circularization onboard the instrument as part of the run.

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SpecificationAdept Library Compatibility Kit v1.1Adept Rapid PCR-Plus Kit

Kit size

24 reactions

24 reactions

Indexing

≤ 10 bp dual index

≤ 10 bp dual index

UMIs

Supported

Supported
Storage-25°C to -15°C-25°C to -15°C
SequencingAVITIAVITI
Adept Standard Protocol
Input/reaction0.2–0.5 pmolNot applicable
Total time~75 minutesNot applicable
Hands-on time~25 minutesNot applicable
Adept PCR-Free Protocol
Input/reaction0.1–0.5 pmolNot applicable
Total time~40 minutesNot applicable
Hands-on time~10 minutesNot applicable
Adept PCR-Plus Protocol
Input/reactionNot applicable0.05 pmol
Total timeNot applicable~40 minutes
Hands-on timeNot applicable~20 minutes

Catalog Numbers

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ProductCatalog #

Element Adept Library Compatibility Kit v1.1

830-00007

Adept Rapid PCR-Plus Kit

830-00018

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