Element Elevate™ Library Prep Workflow
The Element Elevate Library Prep Workflow offers native library prep solutions for a wide range of applications including whole-genome sequencing (WGS) and other DNA sequencing applications. Built on a foundation of efficient recovery and consistent results, the Elevate Workflow accommodates variable sample types and input amounts, including low input and low quality. Efficient, user-friendly protocols with onboard circularization minimize touchpoints and accelerate library prep time.
Go End-to-End with Elevate
The Elevate Workflow features a modular kit design for greater flexibility. Mix and match library prep, enrichment, and index kits to configure your ideal end-to-end prep. Workflow choices include:
- DNA library prep with or without PCR
- Mechanical or enzymatic fragmentation of DNA samples
- Multiplexing of up to 96 samples with 96 unique dual indexes (UDIs) for accurate read assignment
- Standalone index and adapter plates for custom library prep
- Size selection that calibrates insert sizes
Elevate Specifications
Built to stringent specifications to support a variety of study requirements, DNA and RNA library prep kits prepare libraries for avidite base chemistry sequencing in less than four hours. For complete compatibility information, see Product Compatibility.
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Specification | Mechanical Kit | Enzymatic Kit | RNA Kit |
Kit size | 24 reactions | 24 reactions | 24 reactions |
Multiplexing | ≤ 96 UDIs | ≤ 96 UDIs | ≤ 96 UDIs |
Automation capability | Liquid handling robot | Liquid handling robot | Liquid handling robot |
Input requirement | 1–500 ng sheared genomic (gDNA) | 1–500 ng gDNA | 10–4000 ng RNA |
Fragmentation method | Covaris shearing | Enzymatic | Enzymatic |
Enrichment method | Not applicable | Not applicable | mRNA enrichment or rRNA depletion |
PCR-free protocol time* | 2 hours and 25 minutes | 1 hour and 50 minutes | Not applicable |
PCR-plus protocol time* | 3 hours and 25 minutes | 2 hours and 55 minutes | 6 hours |
Storage | -25°C to -15°C | -25°C to -15°C | -25°C to -15°C |
* Approximate total time that includes hands-on time
Catalog Numbers
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Product | Catalog # |
DNA Library Prep | |
Elevate Mechanical Library Prep Kit | 830-00008 |
Elevate Enzymatic Library Prep Kit | 830-00009 |
Elevate Long UDI Adapter Kit Set A | 830-00010 |
Elevate Index and Adapter Kit | 830-00005 |