Peripheral blood mononuclear cells (PBMCs) comprise a mixture of cells from peripheral blood which include lymphocytes, monocytes, natural killer cells, and dendritic cells.
Based on our internal experience handling PBMCs in our 12-well slide kit, we recommend resuspending cells at 1,000,000 cells/ml, resulting in ~150,000 cells per well. The Teton assay should be run using the Small Cell Recipe option during run setup. In post-run analysis, we expect to see reduced total counts for both RNA and Protein targets compared to adherent cells due to their significantly smaller size.