While targeted sequencing applications have benefitted from innovations in probe design and manufacturing, the workflow has remained unchanged since 2008 — until now.
By drastically simplifying target capture, our Trinity workflow radically reimagines targeted sequencing workflows, at any scale. It saves up to 5 hours of hands-on time by eliminating some steps and automating others on board an AVITI™ sequencer, without compromising quality or cost, ultimately accelerating your time to discovery.
Experience the difference and pre-order your Trinity sequencing kit today.
Rapid data-driven decision making
The traditional hybrid selection process for targeted panels, like exomes, involves a time-consuming process with multiple temperature-controlled washes, PCR-based amplification, and library quality control.
With the simplified Trinity workflow, you’ll hybridize your DNA library to the target probes of interest, wait for the prescribed time, and load it directly on your AVITI sequencer. This drastically reduces manual steps and additional amplification resulting in higher library complexity and lower duplication rates.
Faster workflows without compromising on quality
Using our one-hour fast hybridization workflows, Trinity enables the fastest exome workflow on the market today with turnaround times as little as one day. While Trinity radically changes the targeted sequencing workflow—it doesn’t compromise data quality. Download our infographic and see how in comparison studies, the Trinity workflow demonstrated a lower duplication rate and improved library diversity compared to conventional in-solution workflows. |  |
Compatible Panels
At product release, we have established supported workflows for exome sequencing applications in collaboration with our partners, Twist Bioscience and IDT. To perform this workflow, reagents are purchased from both Element and one of our partners. Trinity sequencing kits and binding reagents can be purchased directly from Element Biosciences while reagents for library preparation and hybridization can be purchased from either of our partners.
Trinity sequencing kits are configured for sequencing 24 exomes per flow cell with mean target coverage at ≥50x and the ability to scale the plexity depending on your coverage needs. For added TAT flexibility, Trinity is available in both 2x75 and 2x150 options.
Exome sequencing is our first supported application, but with some optimization Trinity sequencing kits are compatible with panels ranging from hundreds to thousands of targets. We’ll continue to expand our guidance on running smaller or custom panels and ultimately, enable fully on-instrument target capture.
Explore the Trinity protocols:
IDT xGen Exome & Trinity Quick Guide
Twist Exome & Trinity Quick Guide
Twist Fast Hybridization & Trinity Quick Guide
Performance Metrics
Quality | ≥90% Q30 |
On-Target % | ≥85% |
Fold 80 | Fold 80 ≤1.5 |
Run Time | 2x75: 24 hours 2x150: 38 hours |
Mean Target Coverage (24-plex) | 2x75: ≥30x 2x150: ≥50x |
Ordering Information
Element Biosciences | |
Trinity™ 2x75 Sequencing Kit | 860-00019 |
Trinity™ 2x150 Sequencing Kit | 860-00020 |
Trinity™ Binding Reagent, 12 reactions | 830-00029 |
Trinity™ Fast Hyb Loading Buffer | 830-00030 |
IDT* | |
10022463 | |
Twist Bioscience* | |
Twist for Element Trinity, Exome 2.0 + Comp Spike-in, Standard Hyb Workflow, 192 Samples | 109327 |
Twist for Element Trinity, Exome 2.0 + Comp Spike-in, Fast Hyb Workflow, 192 Samples | 109329 |
*Please contact our partners directly to purchase these reagents.